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Active Ras-GTP

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品牌: NewEastBio
货号: 26909
规格: 30 μL
单价: 4800.00元/支
起订: 1 支
供货总量: 30000 支
发货期限: 自买家付款之日起 1 天内发货
所在地: 湖北 武汉
有效期至: 长期有效
最后更新: 2017-11-10 10:55
浏览次数: 36
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产品详细说明
Active Ras-GTP [26909]
[30 ul]
 

Catalog Number:  26909
Gene Symbol:  RAS
Description:   Anti-Active Ras Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen:   Recombinant full length protein of active H-Ras
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. # 26014).
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