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RhoA Activation Assay Kit

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品牌: NewEastBio
货号: 80601
单价: 6800.00元/kit
起订: 1 kit
供货总量: 30000 kit
发货期限: 自买家付款之日起 1 天内发货
所在地: 湖北 武汉
有效期至: 长期有效
最后更新: 2017-11-10 10:55
浏览次数: 6
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公司基本资料信息
  • 武汉纽斯特生物
  •   [诚信档案]
  • 已缴纳 0.00 元保证金
  • 联系人吴雨(先生)      
  • 会员 [当前离线] [加为商友] [发送信件]
  • 邮件neweastbio2033@163.com
  • 电话027-87561033
  • 手机13871237297
  • 地区湖北-武汉
  • 地址湖北省武汉市东湖开发区光谷生物城创新基地B3-3栋3楼
 
 
产品详细说明
 
RhoA Activation Assay Kit [80601]
[20 Assays]
 

Product Highlight

  • Configuration-specific monoclonal antibody that recognizes RhoA-GTP, but not RhoA-GDP
  • The only available RhoA activation assay kit directly measures the level of RhoA-GTP
  • Providing reliable results with consistent reproducibility than other RhoA activation assay kits based on certain downstream signaling pathways
  • Providing shorter experimental time
  • Could be used to monitor the activation of RhoA in cells and in tissues by immunohistochemistry

Product Description

    Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of RhoA, and the hydrolysis of GTP to GDP renders it inactive.

    Currently the activation of RhoA proteins is assayed with the binding of GTP-bound RhoA to the RhoA-binding domain (RBD) of the RhoA effector protein, Rhotekin. This method is based on the observation that the active, GTP-bound RhoA could bind to the RBD of rhotekin. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of RBD to RhoA-GTP.

    NewEast Biosciences RhoA Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes RhoA-GTP, but not RhoA-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time. This assay provides the reliable results with consistent reproducibility.

    This anti-RhoA-GTP monoclonal antibody can also be used to monitor the activation of RhoA in cells and in tissues by immunohistochemistry.

    NewEast Biosciences RhoA Activation Assay Kit provides a simple and fast method to monitor the activation of RhoA. Each kit provides sufficient quantities to perform 20 assays.

     

Assay Principle

    NewEast Biosciences RhoA Activation Assay Kit bases on the configuration-specific anti-RhoA-GTP monoclonal antibody to measure the active RhoA-GTP levels, either from cell extracts or from in vitro GTPγS loading RhoA activation assays. Briefly, anti-active RhoA mouse monoclonal antibody will be incubated with cell lysates containing RhoA-GTP. The bound active RhoA will then be pulled down by protein A/G agarose. The precipitated active RhoA will be detected by immunoblot analysis using anti-RhoA rabbit polyclonal antibody.

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