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Cdc42 Activation Assay Kit

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品牌: NewEastBio
货号: 80701
单价: 6800.00元/kit
起订: 1 kit
供货总量: 30000 kit
发货期限: 自买家付款之日起 1 天内发货
所在地: 湖北 武汉
有效期至: 长期有效
最后更新: 2017-11-10 10:55
浏览次数: 16
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  • 武汉纽斯特生物
  •   [诚信档案]
  • 已缴纳 0.00 元保证金
  • 联系人吴雨(先生)      
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  • 邮件neweastbio2033@163.com
  • 电话027-87561033
  • 手机13871237297
  • 地区湖北-武汉
  • 地址湖北省武汉市东湖开发区光谷生物城创新基地B3-3栋3楼
 
 
产品详细说明
 
Cdc42 Activation Assay Kit [80701]
[20 assays]
 

Product Highlight

  • Configuration-specific monoclonal antibody that recognizes Cdc42-GTP, but not Cdc42-GDP
  • The only available Cdc42 activation assay kit directly measures the level of Cdc42-GTP
  • Providing reliable results with consistent reproducibility than other Cdc42 activation assay kits based on certain downstream signaling pathways
  • Providing shorter experimental time
  • Could be used to monitor the activation of Cdc42 in cells and in tissues by immunohistochemistry

    Product Description

    Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive.

    Currently the activation of Cdc42 proteins is assayed with the binding of GTP-bound Cdc42 to the p21-binding domain (PBD) of p21-activated protein kinase (PAK). This method is based on the observation that the active, GTP-bound Cdc42 (and Rac) could bind to the PBD of PAK. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of PBD to Cdc42-GTP.

    NewEast Biosciences Cdc42 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Cdc42-GTP, but not Cdc42-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time. This assay provides the reliable results with consistent reproducibility.

    These anti-Cdc42-GTP monoclonal antibody can also be used to monitor the activation of Cdc42 in cells and in tissues by immunohistochemistry.

    NewEast Biosciences Cdc42 Activation Assay Kit provides a simple and fast method to monitor the activation of Cdc42. Each kit provides sufficient quantities to perform 20 assays.

    Assay Principle

    NewEast Biosciences Cdc42 Activation Assay Kit bases on the configuration-specific anti-Cdc42-GTP monoclonal antibody to measure the active Cdc42-GTP levels, either from cell extracts or from in vitro GTPγS loading Cdc42 activation assays. Briefly, anti-active Cdc42 mouse monoclonal antibody will be incubated with cell lysates containing Cdc42-GTP. The bound active Cdc42 will then be pulled down by protein A/G agarose. The precipitated active Cdc42 will be detected by immunoblot analysis using anti-Cdc42 rabbit polyclonal antibody.

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