QQ:859207209
标王 热搜: 技术  生物  TNF  FGF  色谱  MIP  试剂  仪器设备  离心机  仪器 
 

增强型绿色荧光蛋白EGFP

点击图片查看原图
品牌: PuriMag
单价: 面议
起订:
供货总量:
发货期限: 自买家付款之日起 3 天内发货
所在地: 福建省 厦门
有效期至: 长期有效
最后更新: 2018-09-09 17:45
浏览次数: 22
询价
公司基本资料信息
 
 
产品详细说明
 Name: EGFP (Enhanced Green Fluorescent Protein Recombinant)

Pricings: 5μg (RMB 300.00), 20μg (RMB 780.00), 1mg (RMB 11400.00)

Shipped: At Room Temperature

Catalogue Number: PRO-0001

SYNONYMS

Green fluorescent protein, GFP.

INTRODUCTION

GFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. GFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of proteinprotein interactions. Enhanced GFP (eGFP) has F64L and S65T mutations, which make GFP show increased fluorescence and fold more efficiently under 37 .

DEscriptION

Recombinant EGFP produced in E.coli cells is a nonglycosylated, homodimeric protein containing 239 amino acid chain and having a molecular mass of 26.9kDa. EGFP is purified by proprietary chromatographic techniques.

SOURCE

Escherichia Coli.

 

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freezedried) powder.

 

FORMULATION

The EGFP was lyophilized from a 0.2μm filtered concentrated solution in PBS pH 7.4.

 

SOLUBILITY

It is recommended to reconstitute the lyophilized EGFP in sterile distilled H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

 

STABILITY

Lyophilized EGFP although stable at room temperature for 3 weeks, should be stored desiccated below 18℃. Upon reconstitution EGFP should be stored at 4℃ between 2-7 days and for future use below 18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freezethaw cycles.

 

PURITY

Greater than 95.0% as determined by: (a) Analysis by RPHPLC. (b) Analysis by SDSPAGE.

 

AMINO ACID SEQUENCE

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTRAEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMVLLEFVTAAGI TLGMDELYK

 

USAGE

PuriMag's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

0条 [查看全部]  相关评论
 
更多»本企业其它产品

[ 供应搜索 ]  [ 加入收藏 ]  [ 告诉好友 ]  [ 打印本文 ]  [ 违规举报 ]  [ 关闭窗口 ]

 
网站首页 | 广告图文 | 付款方式 | 关于我们 | 版权隐私 | 联系方式 | 诚聘英才 | 网站地图 | 排名推广 | 广告服务 | 积分换礼 | 网站留言 | RSS订阅 | 京ICP备05052705号
Powered by DESTOON